Laboratory for Molecular Photonics

Department of Chemistry

University of Miami

Goal: The goal of this project is the development of photoactivatable fluorophores designed to permit the acquisition of fluorescence images with spatial resolution at the nanometer level.

Results: The Laboratory of Molecular Photonics develops fluorophore-photochrome dyads designed to switch from a nonemissive to an emissive state reversibly upon illumination at an appropriate activation wavelength and permit the imaging of polymer nanoparticles and cellular substructures with subdiffraction resolution.

Keywords: Coumarins Fluorescence Halochromism Photochromism Molecular Switches Oxazines Photoactivatable Fluorophores Superresolution Imaging

Selected Articles

 "Photoactivatable Fluorophores for Super-Resolution Imaging Based on Oxazine Auxochromes": E. Deniz, M. Tomasulo, J. Cusido, I. Yildiz, M. Petriella, M. L. Bossi,

The reversible photoactivation of a coumarin fluorophore with the interconversion of a photochromic oxazine allows the imaging of the tubulin structure of model cells with subdiffraction resolution.

     Sortino, F. M. Raymo, J. Phys. Chem. C, 2012, 116, 6058–6068

 "Fast Fluorescence Switching within Hydrophilic Supramolecular Assemblies": J. Cusido, M. Battal, E. Deniz, I. Yildiz, S. Sortino, F. M. Raymo, Chem. Eur. J., 2012, 18, 10399–10407

 "Fluorescence Photoactivation by Intermolecular Proton Transfer": S. Swaminathan, M. Petriella, E. Deniz, J. Cusido, J. D. Baker, M. L. Bossi, F. M. Raymo, J. Phys. Chem. A, 2012, 116, 9928–9933

 "A Photochromic Bioconjugate with Photoactivatable Fluorescence for Superresolution Imaging": J. Cusido, S. Shaban Ragab, E. R. Thapaliya, S. Swaminathan, J. Garcia-Amorós, M. J. Roberti, B. Araoz, M. M. A. Mazza, S. Yamazaki, A. M. Scott, F. M. Raymo, M. L. Bossi, J. Phys. Chem. C, 2016, 120, 12860–12870

Selected Reviews

  "Molecular Strategies to Read and Write at the Nanoscale with Far-Field Optics": J. Cusido, S. Impellizzeri, F. M. Raymo, Nanoscale, 2011, 3, 59–70

 "Photoactivatable Synthetic Dyes for Fluorescence Imaging at the Nanoscale": F. M. Raymo, J. Phys. Chem. Lett., 2012, 3, 2379–2385