James N. Wilson

ASSOCIATE PROFESSOR

344 Cox Science Center
1301 Memorial Drive, Coral Gables, FL 33146

E-mail: jnwilson@miami.edu

Office:

(305) 284-2619

Lab:

(305) 284-8557

AREAS OF FOCUS

Bioorganic and Materials Chemistry

RESEARCH INTERESTS

Research in my group is in the general areas of bioorganic and materials chemistry with a specific focus on the development of fluorescent analogs of biomolecules. We design, synthesize and characterize probes targeting specific biomacromolecular structures including DNA, transmembrane proteins, enzymes and receptors. This effort incorporates several research activities including quantum chemical calculations, organic synthesis, optical spectroscopy, in vitro cellular studies, and fluorescence microscopy.

Fluorescent analogs of tyrosine kinase inhibitors allow tracking of drug distribution, aggregation and binding in cancer cells.  These dynamics can be linked to cellular outcomes (survival, reduced proliferation, or death) improving our understanding of drug resistance mechanisms

EDUCATION AND PROFESSIONAL EXPERIENCE

2008-present, Assistant Professor, Department of Chemistry University of Miami
2007-2008, Visiting Assistant Professor, Department of Chemistry, University of San Francisco
2005-2007, Postdoctoral Fellow, Stanford University
2004, Ph.D. in Organic Chemistry, Georgia Institute of Technology
2000, B.S. in Chemistry, University of South Carolina

AWARDS AND FELLOWSHIPS
  • 2010-11, American Cancer Society, IRG Awardee
  • 2005-07, Ruth L. Kirschstein NIH Postdoctoral Fellowship
GRANTS
  • 2013 National Cancer Institute, IMAT Program
  • 2012 Bankhead-Coley Cancer Research Program
  • 2011 James & Esther King Research Program
  • 2010 American Cancer Society, Junior Faculty Development Grant
  • 2010 University of Miami Interdisciplinary Research Development Initiative
  • 2010 University of Miami Provost Award
  • 2009 University of Miami Provost Award
  • 2007 NIH/NIGMS F32 GM075697 
SELECTED PUBLICATIONS
  • Wilson, J. N.; Wigenius, J.; Pitter, D. R. G.; Qiu, Y.; Abrahamsson, M.; Westerlund, F. Base Pair Sensitivity and Enhanced ON/OFF Ratios of DNA-Binding: Donor−Acceptor−Donor Fluorophores, J. Phys. Chem. B, 2013117, 12000-12006.
  • Pitter, D. R. G.; Wigenius, J.; Brown, A. S.; Baker, J. D.; Westerlund, F.; Wilson, J. N. Turn-On, Fluorescent Nuclear Stains with Live Cell Compatibility, Org. Lett.201315, 1330-1333.
  • (Highlighted in C&E News, "A Bright Idea for a Nucleus Stain" March, 2013)       (Included in the ACS virtual issue: Nucleic Acids - Chemistry and Applications)
  • Adjaye-Mensah, E.; Gonzalez, W. G.; Miksovska, J.; Wilson, J. N. Photophysical Characterization of a Benzo-Fused Analogue of Brooker’s Merocyanine: Solvent Polarity and pH Effects, J. Phys. Chem. A2012116, 12470.
  • Wilson, J. N.; Brown, A. S.; Babinchak, W. M.; Ridge, C. D.; Walls, J. D. Fluorescent Stilbazolium dyes as probes of the norepinephrine transporter: structural insights into substrate binding, Org. Biomol. Chem. 201210, 8710.
  • Sicard, R.; Dhuguru, J.; Liu, W.; Patel, N.; Landgraf, R.; Wilson, J. N. A fluorescent reporter of ATP binding-competent receptor kinases, Bioorg. Med. Chem. Lett., 201222, 5532.
  • Smith, E. L.; Brown, A. S.; Adjaye-Mensah, E.; Wilson, J. N. “Probing the functional limits of the norepinephrine transporter with self-reporting, fluorescent stilbazolium dimers.” Org. Biomol. Chem., 201210, 1493.
  • Dhuguru, J.; Gheewala, C.; Kumar, N. S. S.; Wilson, J. N. “Highly Chromic, Proton Responsive Phenyl Pyrimidones.” Org. Lett., 201113, 4188-4191.
  • Bagale, M. D.; Brown, A. S.; Carballosa Gonzalez, M. M.; Vitores, A.; Micotto, T. L.; Kumar, N. S. S.; Hentall, I. D.; Wilson, J. N. “Fluorescent reporters of monoamine transporter distribution and function.” Bioorg. Med. Chem. Lett.,201121, 7387-7391.
  • Gujrati, M. D.; Kumar, N. S. S.; Brown, A. S.; Captain, B.; Wilson, J. N. “Luminescent Charge-Transfer Complexes: Tuning Emission in Binary Fluorophore Mixtures.”, Langmuir201127, 6554-6558.
  • Brown, A. S.; Bernal, L. -M.; Micotto, T. L.; Smith, E. L.; Wilson, J. N.  “Fluorescent Neuroactive Probes Based on Stilbazolium Dyes.” Org. Biomol. Chem.20119, 2142-2148.
  • Kumar, S. N. S.; Gujrati, M. D.; Wilson, J. N. “Evidence of preferential ?-stacking: a study of intermolecular and intramolecular charge transfer complexes.” Chem. Commun. 201046, 5464-5466.
  • Micotto, T. L.; Brown, A. S.; Wilson, J. N. “Fluorescent mimics of 5-hydroxytryptamine based on N-alkylated derivatives of 6-hydroxycarbostyril.” Chem. Commun. 2009, 7548-7550.