T. K. Harris

Yildiz, I., Gao, X., Harris, T. K., and Raymo, F. M. (2008) Fluoresence resonance energy transfer in quantum dot-protein kinase assemblies. J. Biomed. Biotech. (in press).

Keshwani, M. M., Ross, D. B., Ragan, T. J., and Harris, T. K. (2008) Baculovirus-mediated expression, purification, and characterization of a fully activated catalytic kinase domain of the 70 kDa 40S ribosomal protein S6 kinase-1 aII isoform (S6K1aII). Protein Expr. Purif. (in press, doi:10.1016/j.pep.2007.11.003).

Ragan, T. J., Ross, D., Keshwani, M., and Harris, T. K. (2008) Expression, purification, and characterization of a structurally disordered but functional C-terminal autoinhibitory domain (AID) of the 70 kDa 40S ribosomal protein S6 kinase-1 (S6K1). Protein Expr. Purif. 57, 271-279.

Al-Ali, H., Ragan, T. J., and Harris, T. K. (2007) Reconstitution of modular PDK1 functions on trans-splicing of the regulatory PH and catalytic kinase domains. Bioconjugate Chem. 18, 1294-1302.

Harris, T. K. (2006) Discovering new drug targeting sites on flexible multi-domain protein kinases: combining segmental isotopic and site-directed spin labeling for NMR detection of interfacial clefts. Methods Mol. Biol. 316, 199-225.

Gao, X., and Harris, T. K. (2006) Steady-state kinetic mechanism of PDK1. J. Biol. Chem. 281, 21670-21681.

Gao, X., and Harris, T. K. (2006) Role of PH domain in regulating in vitro autophosphorylation events required for reconstitution of PDK1 catalytic activity. Bioorg. Chem. 34, 200-223.

Gao, X., Yo, P., and Harris, T. K. (2005) Improved yields for baculovirus-mediated expression of human His6-PDK1 and His6-PKBb/Akt2 and characterization of phospho-specific isoforms for design of inhibitors that stabilize inactive conformations. Protein Expr. and Purif. 43, 44-56.

Harris, T. K. (2005) Discovering new drug targeting sites on flexible multi-domain protein kinases: combining segmental isotopic and site-directed spin labeling for NMR detection of interfacial clefts. Methods Mol. Biol. 316, 199-225.

Gao, X., Yo, P., Keith, A., Ragan, T. J., and Harris, T. K. (2003) Thermodynamically balanced inside-out (TBIO) PCR-based gene synthesis: a novel method of primer design for high-fidelity assembly of longer gene sequences. Nuc. Acids Res. 31(22), e143.

Harris, T. K. (2003) PDK1 and PKB/Akt: ideal targets for development of new strategies to structure-based drug design. IUBMB Life 55, 117-126.

Harris, T. K., and Turner, G. J. (2002) Structural basis for perturbed pKa values of catalytic residues in enzyme active sites.  IUBMB Life 53, 85-98.

 

 
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